Western Blot
Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: HA-tagged fusion protein
All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution

Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).